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Palmitate in vitro induces a hyper-metabolic phenotype in B cells from both YL and EL individuals. Total B cells from the same YL and EL individuals in Figures 3, 4 were stimulated with CpG, alone or in the presence of palmitate for 6 h, and evaluated for the mRNA expression of HK-2, LDHA, PDHX and ACACB. Heatmap shows qPCR values (2−ΔCt) of metabolic markers, normalized to GAPDH, from four individuals/group.
