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. 2022 Feb 14;3:800153. doi: 10.3389/fragi.2022.800153

FIGURE 4.

FIGURE 4

(A) Quantitative real-time PCR of Lip3 transcript in isolated adult brains from fed (0 h) and starved (2, 24, 48 h) male (blue squares) and female (red squares) normalized to rp49 and actin-5c housekeeping genes and relative to whole body from fed male and female flies, respectively. (B) Quantitative real-time PCR of Lip3 transcript in isolated adult brains from male (blue squares) and female (red squares) flies, normalized to rp49 and actin-5c housekeeping genes and relative to whole body from 5 day old male and female flies, respectively. Datasets for fed flies in A and young flies in B are identical. (C) Representation of the CDP-DAG pathway of PI synthesis. (D) Quantitative real-time PCR of DAG kinase (Dgk) transcript in isolated adult brains, normalized to rp49 and actin-5c housekeeping genes and whole body from 5 day old flies (male brains normalized to male whole flies and female brains normalized to female whole body). (E) Quantitative real-time PCR of phosphatidate cytidyltransferase (Cds) transcript in isolated adult brains, normalized to rp49 and actin-5c housekeeping genes and whole body from 5 day old flies (male brains normalized to male whole flies and female brains normalized to female whole body). (F) Quantitative real-time PCR of CDP-diacylglycerol-inositol-3-phosphatidyl-transferase (PIS) transcript in isolated adult brains, normalized to rp49 and actin-5c housekeeping genes and whole body from 5 day old flies (male brains normalized to male whole flies and female brains normalized to female whole body). Asterisks represent *p < 0.05, ***p < 0.001.