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. 2022 Feb 2;2:803482. doi: 10.3389/fragi.2021.803482

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Copyright © 2022 Chen, De Angulo, Guo, More, Ochsner, Lopez, Saul, Pang, Sun, McKenna and Tong.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Generation of Adipocyte-Specific PU.1 KO Mice. (A) The schematic diagram of AdipoqCre-PU.1^fl/fl Mice. Mice that have the PU.1 gene exon 5 flanked with loxP sequences were breed with mice carrying a transgene containing Cre recombinase under the control of Adiponectin gene promoter and enhancer. (B) The expression of PU.1 in isolated adipocytes was detected using anti-PU.1 Western blot analysis.