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. 2022 Feb 2;2:803482. doi: 10.3389/fragi.2021.803482

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Copyright © 2022 Chen, De Angulo, Guo, More, Ochsner, Lopez, Saul, Pang, Sun, McKenna and Tong.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Adipocyte PU.1 Deficiency Has No Effect on Body Weight, Body Composition and Glucose Tolerance in Young Adult Male Mice. (A) Body weight (BW) and body composition of young adult (4–5 months of age) male AdipoqCre-PU.1^fl/fl mice (N = 8) and the control littermate PU.1^fl/fl mice (N = 8). (B) For glucose tolerance test, mice (N = 8 vs. 8) were fasted overnight and injected with glucose. Blood glucose was then measured. (C) Energy expenditure was determined using indirect calorimetry in the integrated Comprehensive Lab Animal Monitoring System (CLAMS; Columbus Instruments) in 4–5 months old male AdipoqCre-PU.1^fl/fl mice (N = 8) and the control littermate PU.1^fl/fl mice (N = 8). (D) Total locomotor activity under fed or fasting condition. *p < 0.05, **p < 0.01.