TABLE 1.

Real-time PCR primers.

Primers
Gene		Sequence (5′-3′)
TNF-α	Forward	ATG​GCC​TCC​CTC​TCA​TCA​GT
	Reverse	TGG​TTT​GCT​ACG​ACG​TGG​G
IL-6	Forward	TCC​GGA​GAG​GAG​ACT​TCA​CA
	Reverse	TTG​CCA​TTG​CAC​AAC​TCT​TTT​CT
AChRα	Forward	GCC​ATT​AAC​CCG​GAA​AGT​GAC
	Reverse	CCC​CGC​TCT​CCA​TGA​AGT​T
MuSK	Forward	ACC​GTC​ATC​ATC​TCC​ATC​GTG​T
	Reverse	CTC​AAT​GTT​ATT​CCT​CGG​ATA​CTC​C
18s	Forward	GCT​TGC​TCG​CGC​TTC​CTT​ACC​T
	Reverse	TCA​CTG​TAC​CGG​CCG​TGC​GTA
Probes
Gene	Assay ID
p16(INK4a)	Mm00494449_m1 (Thermo Fisher Scientific)