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. 2022 Jan 12;2:797562. doi: 10.3389/fragi.2021.797562

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Copyright © 2022 Noh, Blasco-Conesa, Lai, Ganesh, Urayama, Moreno-Gonzalez, Marrelli, McCullough and Moruno-Manchon.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cultured CEC from aged mice showed higher levels of RNA-G4 than cultured young CEC. (A) CEC were isolated from young (4-m/o) and aged (20-m/o) female mice and cultured. Cells were fixed and stained with antibodies against G4 (BG4) anti against actin, and with the nuclear Hoechst dye. (B) Cultured CEC from young and aged female mice were fixed and incubated with DNaseI to eliminate DNA G4, and then stained with antibodies against G4 (BG4) and against actin, and with the nuclear Hoechst dye. (C,D) BG4 fluorescence intensities were measured. (C) Quantification from (A). Scale bar (Yg), 20 μm; Scale bar (Ag), 40 μm. t-test, *p-value = 0.0116. (D) Quantification from (B). t-test, *p-value = 0.0435. Data were pooled from three independent experiments analyzing 50 cells per condition and experiment.