FIGURE 1.

GCN2 function is essential for development under restriction of individual EAA except for methionine. (A) Egg-to-adult viability of w ^Dah control and Gcn2 ¹ mutants on chemically defined Yaa and HUNTaa diets. (B) Relative proportion of EAA in the Yaa diet compared to the HUNTaa diet. Arginine (R) highlighted in blue was identified as the most limiting EAA in HUNTaa. (C) Decreasing R in the Yaa diet by 70% (−R) reduced viability of Gcn2 ¹ mutants. Supplementation of R to the HUNTaa (+R) diet rescued viability of Gcn2 ¹ mutants. (D) Increasing the absolute amount of all aa by 50% in HUNTaa did not rescue viability of Gcn2 ¹ mutants. (E) Viability of w ^Dah control and Gcn2 ¹ mutants on Yaa diets with individual EAA restriction by 70%. (F) Viability of flies upon restriction of W and M by 80% and 90%. Viability (%) represents the percentage of eclosed adult flies per fly embryo. n = 10 vials with 25 embryos per vial. Median, 25th and75th percentiles, and Tukey whiskers are indicated. Two-way ANOVA followed by Bonferroni’s post-hoc test, p values were adjusted for multiple comparisons.