FIGURE 2.
GCN2 function is essential for survival under deprivation of individual EAA except for methionine. (A–K) Survival of w Dah control and Gcn2 1 mutant female flies under full deprivation of individual EAA (A–J) and all amino acids (-All) (K). Gcn2 1 mutants showed significantly increased lifespan compared to w Dah flies on the Yaa diet (p = 7.8 × 10−3, log-rank test). Survival of Gcn2 1 mutants was significantly reduced compared to w Dah on -R (p = 6.0 × 10−73) (A), -L (p = 2.9 × 10−41) (B), -I (p = 9.9 × 10−63) (C), -V (p = 1.9 × 10−64) (D), -F (p = 1.3 × 10−67) (E), -H (p = 2.4 × 10−25) (F), -K (p = 1.4 × 10−12) (G), -T (p = 5.3 × 10−15) (H), -W (p = 3.6 × 10−72) (I) diets, log-rank test. In contrast, Gcn2 1 mutants were significantly longer lived than w Dah control flies on M deprived medium (p = 4.4 × 10−25, log-rank test) (J). Under deprivation of all amino acids (K), Gcn2 1 mutants had a similar lifespan than w Dah flies (p = 0.66, log-rank test). Survival curves of w Dah and Gcn2 1 mutants on the Yaa diet are the same in (A–K). (L) Summary of survival analysis represented as percentage change in median lifespan of Gcn2 1 mutants relative to w Dah controls on each diet. n = 150 flies for each condition of each genotype.