FIGURE 3.

GCN2 regulates feeding behaviour under long-term but not short-term deprivation of individual EAA. (A–C) Feeding rates measured by proboscis extension assay of w ^Dah control and Gcn2 ¹ mutant females over a period of 7 days on Yaa, -L, -R, -M, and -All diets (A). Feeding rates of w ^Dah controls and Gcn2 ¹ mutants on day 1 (B) and day 4 (C). (D,E) Feeding rates of w ^Dah controls and Gcn2 ¹ mutants on V, I, F, H, K, T, and W deprived diets on day 1 (D) and day 4 (E). n = 6 vials with five flies per vial. Data are present as mean ± s.e.m. One-way ANOVA followed by Dunnett’s multiple comparisons test, p values were adjusted for multiple comparisons.