FIG. 4.
DGGE profiles of B. adolescentis E-981074T 16S PCR product and its derivative clones. (a) Heteroduplex formation experiment by PCR. Templates used in PCR are as follows: lane 1, plasmid from clone b1; lane 2, plasmid from clone c1; lane 3, plasmids from clones b1 and c2; lane 4, DNA from E-981074T. (b) Heteroduplex formation experiment by melting PCR products. PCR products are as follows: lane 5, clone b1; lane 6, clone c1; lane 7, clones b1 and c1 heat denaturated together and cooled slowly to allow reannealing of complementary strands; lane 8, E-981074T. Single-stranded DNA is indicated with an arrowhead.