TABLE 2.

Primers used in the present study

Primer	Sequence (5′ to 3′)	Use	Specificity or target	Reference
Bif164-f	GGGTGGTAATGCCGGATG	PCR	Bifidobacterium 16S	17, 19
Bif662-r	CCACCGTTACACCGGGAA	PCR	Bifidobacterium 16S	17, 19
Bif164-GC-f	CGCCCGGGGCGCGCCCCGGGCGGGGCGGGGGCACGGGGGG-GGGTGGTAATGCCGGATG	PCR	Bifidobacterium 16S	This study
Bif662-GC-r	CGCCCGCCGCGCGCGGCGGGCGGGGCGGGGGCACGGGGGG-CCACCGTTACACCGGGAA	PCR	Bifidobacterium 16S	This study
Im26-f	GATTCTGGCTCAGGATGAACG	PCR	Bifidobacterium 16S	15
Im3-r	CGGGTGCTICCCACTTTCATG	PCR	Bifidobacterium 16S	15
7-f	AGAGTTTGAT(C/T)(A/C)TGGCTCAG	PCR	Eubacterial 16S	18
1510-r	ACGG(C/T)TACCTTGTTACGACTT	PCR	Eubacterial 16S	18
1401-r	CGGTGTGTACAAGACCC	RT-PCR	Eubacterial 16S	30
338-r	CTGCTGCCTCCCGTAGGAGT	Sequencing	Eubacterial 16S	18
338-f	CTCCTACGGGAGGCAGCAG	Sequencing	Eubacterial 16S	18
515-r	ATCGTATTACCGCGGCTGCTGGCAC	Sequencing	Eubacterial 16S	18
968-f	AACGCGAAGAACCTTA	Sequencing	Eubacterial 16S	18
1100-r	GGGTTGCGCTCGTTG	Sequencing	Eubacterial 16S	18
T7	TAATACGACTCACTATAGGG	Sequencing	pGEMT	Promega
Sp6	GATTTAGGTGACACTATAG	Sequencing	pGEMT	Promega