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. 2022 Apr 4;3:864860. doi: 10.3389/fragi.2022.864860

FIGURE 2.

FIGURE 2

Aging causes decreases in collecting lymphatic vessel density and collecting LEC numbers and alters their phenotype. (A) Representative confocal images of mouse skin collecting lymphatic vessels showing colocalization of PDPN and α-SMA. Quantification of collecting lymphatic vessel (B) density and (C) diameter. (D) Representative flow cytometry dot plots showing frequencies of CD31+/CD45-/PDPN+/LYVE-1- collecting LECs. (E) Quantification of the absolute number of skin collecting vessel LECs among 100,000 total cells. (F) Representative high magnification confocal images of young and aged collecting lymphatic vessels showing orientation of LMCs (α-SMA) relative to LECs (PDPN). (G) Representative high magnification confocal images of young and aged collecting lymphatic vessels showing expression of ryanodine receptor 1 (RyR1) in LMCs (α-SMA). (H) Quantification of RyR1 density as a percentage of LMC density. n = 5 mice per group for all experiments. All quantifications are mean ± SD, unpaired Student’s t test.