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. 2022 Apr 4;3:864860. doi: 10.3389/fragi.2022.864860

FIGURE 6.

FIGURE 6

Aging causes decreased chemokine and altered junctional protein expression by LECs. (A) Representative confocal images of ear skin sections stained for lymphatic vessels (LYVE-1) and CCL21. (B) Quantification of CCL21 immunostaining as a percentage of lymphatic vessel area. (C) qRT-PCR quantification of CCL21 mRNA expression in sorted skin LECs. (D) Representative confocal images of ear skin wholemount stained for nuclei (DAPI), VE-cadherin (upper panel, Initial lymphatics; lower, collecting lymphatics). (E) qRT-PCR quantification of VE-cadherin and (F) ZO-1 mRNA expression in sorted skin LECs. (G) Representative confocal images of ear skin sections stained for lymphatic vessels (LYVE-1) and ICAM-1 (upper panel, immunofluorescence of both; lower, ICAM-1 only). (H) qRT-PCR quantification of mRNA expression of endothelial adhesion molecules ICAM-1 and (I) p-Selectin from sorted skin LECs. n = 5 mice from each group. All quantifications are mean ± SD, unpaired Student’s t test.