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. 2022 Apr 28;3:796087. doi: 10.3389/fragi.2022.796087

FIGURE 3.

FIGURE 3

TrkB-Fc Significantly Elevates H3K9me3 in 12 and 21 DIV Hippocampal Neuron Cultures. 12 and 21 DIV were compared to 6 DIV. Relative H3K9me3 levels are normalized to untreated controls for each culture age. (A) H3K9me3 western blot analysis of hippocampal neuron cultures treated with 1 μg/ml TrkB = TrkB-Fc for 24 h. Welch’s ANOVA, *p = 0.015; n = 7 for 6 DIV, n = 9 for 12 DIV, n = 5 for 21 DIV. Dunnet’s T3 multiple comparisons test, *p = 0.018 6 DIV vs. 21 DIV, *p = 0.048 6 DIV vs. 12 DIV. (B) H3K9me3 western blot analysis of hippocampal neuron cultures treated with 200 μM H2O2 for 4 h. Welch’s ANOVA, p = 0.12; n = 7 for 6 DIV, n = 9 for 12 DIV, n = 5 for 21 DIV. (C) H3K9me3 western blot analysis of hippocampal neuron cultures treated with 1 μg/ml TrkB-Fc for 24 h and 200 μM H2O2 for 4 h. Welch’s ANOVA, *p = 0.018, n = 7 for 6 DIV, n = 9 for 12 DIV, n = 5 for 21 DIV. Dunnett’s T3 multiple comparisons test, *p = 0.022 6 DIV vs. 12 DIV, p = 0.095 6DIV vs. 21 DIV. (D) H3K9me3 western blot analysis of hippocampal neurons treated with 1 μg/ml TrkB-Fc for 24 h, 200 μM H2O2 for 4 h and antioxidants for 24 h. Welch’s ANOVA, p = 0.44; n = 7 for 6 div, n = 4 for 12 DIV and n = 3 for 21 DIV. (E) H3K9me3 western blot analysis of hippocampal neurons treated with antioxidants for 24 h. Welch’s ANOVA, p = 0.27; n = 7 for 6 DIV, n = 4 for 12 DIV and n = 3 for 21 DIV.