FIGURE 4.
SIRT1 is Elevated in 6 DIV Neurons Treated with Hydrogen Peroxide. (A) SIRT1 western blot analysis of hippocampal neurons treated with 1 μg/ml TrkB-Fc for 24 h. Welch’s ANOVA, p = 0.72. (B) SIRT1 western blot analysis of hippocampal neurons treated with 200 μM H2O2 for 4 h. Welch’s ANOVA *p = 0.011; Dunnett’s T3 multiple comparisons test, *p = 0.037 6 DIV vs. 12 DIV. (C) SIRT1 western blot analysis of hippocampal neurons treated with 1 μg/ml TrkB-Fc for 24 h and 200 μM H2O2 for 4 h. Welch’s ANOVA, p = 0.21. N = 5 for 6 DIV, n = 9 for 12 DIV, n = 4 for 21 DIV.