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. 2022 Jun 23;13:924482. doi: 10.3389/fpls.2022.924482

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Copyright © 2022 Ke, Huang, Viswanath, Hu, Yeh, Mitsuda, Lin and Hsu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Schematic diagrams of the truncated fragments of different deleted versions fused with the GUS reporter gene. The pCAMBIA1391Z vector acts as a negative control that does not have a promoter to drive GUS. The pCAMBIA1301 vector was used as a positive control, and the pCAMBIA NbAGO5 promoter constructs with different lengths of NbAGO5 promoter was created by replacing the 35S promoter (A). LB, left border; CaMV 35S PolyA, cauliflower mosaic virus 35S terminator; HygR, hygromycin resistance gene; 35S promoter, cauliflower mosaic virus 35S promoter; GRP, glycine-rich signal peptide sequence; GUS, β-glucuronidase reporter gene; Nos, nopaline synthase terminator; RB, right border.