FIGURE 4.

Promoter activity of NbAGO5 under normal condition, hormone treatments, and challenge with the virus in Transgenic N. benthamiana. (A) The fluorometric GUS assays were performed with leaves of transgenic N. benthamiana harboring different constructs under normal conditions and (B) hormone treatments. (C) The GUS activities in the plants infiltrated with A. tumefaciens harboring infectious construct of BaMV or expression vector for BaMV-CP or BaMV-TGBp1 were analyzed at 3 dpi. (D) PN4 transgenic N. benthamiana plants were agro-infiltrated with infectious constructs of various potexviruses, including BaMV, PVX, FoMV, and CymMV. The GUS activities were then analyzed at 3 dpi. Each column represents the mean of at least three replicates ± standard error (SE). Statistical significance was analyzed using two-tailed student’s t-tests; *p < 0.05; ^**p < 0.01; ^***P < 0.001. The different lowercase letters above the bars indicate statistically significant differences among the means based on two-way ANOVA followed by Duncan’s multiple range test (P < 0.05).