Table 1.

Common cell cycle markers.

Category	Common markers or reagents
DNA-binding dyes	4′,6-diamidino-2-phenylindole (DAPI), Propidium Iodide (PI), 7-Aminoactinomycin D (7-AAD), Hoechst, DRAQ5™
Proliferation vs. quiescence	Ki67, Mcm2-7, Pyronin Y, p27K
Dead/apoptotic markers	7-AAD, PI, Terminal deoxynucleotidyl transferase (TdT) dUTP Nick-End Labeling (TUNEL), Annexin V, LIVE/DEAD™ stains
Senescence markers	p21, p16Ink4A, Senescence-Associated β-Galactosidase (SAβG)
Nascent DNA labels	BrdU, IdU, CldU, EdU, 3H-Thymidine
G1 phase	CDT1, HDHB (nuclear)
S-phase	SLBP; Formation of PCNA foci; Geminin (present through S-G2)
G2 phase	Cyclin B1 (cytoplasmic); Geminin
M-phase	Phosphorylated Histone H3 (pH3); Cyclin B1 (nuclear); Condensed chromatin as detected by DNA-binding dyes or core histone antibodies