. 2001 Feb;67(2):528–538. doi: 10.1128/AEM.67.2.528-538.2001

TABLE 2.

Heterotrophic alkaliphilic isolates that utilize CNS⁻ as a nitrogen source

Enrichment culture^a	Strain	Oxidation of S₂O₃²⁻ to S₄O₆²⁻	Denitrification	Utilization of NO₃⁻NO₂⁻ as N source	G+C content of DNA, (mol%)	% DNA-DNA homology with^b:
Enrichment culture^a	Strain	Oxidation of S₂O₃²⁻ to S₄O₆²⁻	Denitrification	Utilization of NO₃⁻NO₂⁻ as N source	G+C content of DNA, (mol%)	AG 4	AGJ 1-3
LK	AGCNS 1	+	+	−	64.9	52	81
	AGCNS 2	+	+	+	65.5	58	48
SK	AGCNS 3	−	+	+	65.2	50	76
SS	AGCNS 4	+	−	+	65.4	43	53
	AGCNS 5	+	+	+	65.0	42	67

LK, Kenyan lake sediments; SK, Kenyan soda soils; SS, Siberian soda soils.

The levels of DNA homology for strains AGCNS 1, AGCNS 3, and AGCNS 5 ranged from 70 to 90%, which indicated that these strains belong to the same species; strains AGCNS 2 and AGCNS 4 are less closely related to the other strains (40 to 50% DNA homology). Strains AG 4 and AGJ 1-3 are tetrathionate-forming heterotrophic alkaliphiles isolated previously from the Siberian and Kenyan soda lakes, respectively (35, 36).