TABLE 5.
Parameters of autotrophic growth of thiocyanate-oxidizing alkaliphilic strains with thiocyanate and thiosulfate at pH 10 to 10.2a
| Strain | Maximum specific growth rate
(h−1)
|
Growth yield (mg of protein
mmol−1)
|
S0 formation
|
|||
|---|---|---|---|---|---|---|
| CNS− | S2O32− | CNS− | S2O32− | CNS− | S2O32− | |
| ARh 1 | 0.045 (0.09)b | 0.018 | 8–9 (9.2–11.3) | 6–7 | + | + |
| ARh 2 | 0.015 | 0.08 | 5.9 (6.8) | 4.0 | − | +/−c |
| ARh 3 | 0.015 | 0.07 | 5.7 | 7.5 | − | +/− |
| ARh 4 | 0.010 (0.042) | 0.10 | 4.1 (4.3–6.6) | 5.0 | − | − |
a
Strains ARh 1 and ARh 3 were grown with NH3 as the N source, and strains ARh 2 and ARh 4 were grown with NO3− as the N source.
b
The values in parentheses are values obtained from thiocyanate-limited continuous cultures; strain ARh 1 was grown with 6 mM thiocyanate at pH 10.1, and strain ARh 4 was grown with 10.5 mM thiocyanate at pH 10.2.
c
+/−, variable.