TABLE 7.
Cyanate production by thiocyanate-oxidizing alkaliphilic bacteria at pH 10 after complete thiocyanate utilization
| Strain | Batch
culturea
|
Continuous
cultureb
|
Washed
cellsc
|
|||||
|---|---|---|---|---|---|---|---|---|
| N biomass concn (mM)d | NH3 concn (mM) | CNO− concn (mM) | N biomass concn (mM) | NH3 concn (mM) | CNO− concn (mM) | NH3 concn (mM) | CNO− concn (mM) | |
| ARh 1 | 1.4 | 0.3–0.5 | 10.5–11.5 | 0.6–1.25 | 0.1–1.9 | 4.6–8.5 | 0 | 4.8 |
| ARh 2 | 1.1 | 0.5–1.2 | 11.0–12.0 | 1.10 | 2.0–2.2 | 7.8–8.2 | 0 | 4.8 |
| ARh 4 | 0.9 | 1.2–1.6 | 11.0–12.0 | 0.60–0.72 | 1.25–2.9 | 7.4–9.0 | 0–0.2 | 4.8 |
a
The cultures were grown for 70 to 120 h with 15 mM CNS−.
b
The cultures were grown in CNS−-limited continuous cultures at dilution rates from 0.02 h−1 to 0.09 liter−1, with inflowing concentrations of 6 to 13 mM CNS−. Strain ARh 1 was grown with 6 to 13 mM thiocyanate, strain ARh 2 was grown with 13 mM thiocyanate, and strain ARh 4 was grown with 10.5 to 13 mM thiocyanate.
c
The cultures were incubated for 3 to 4 h with 5.4 mM CNS−.
d
We assumed that the N content of the cell protein is 15%.