TABLE 8.
Cyanase activities in cell extracts prepared from cells of different thiocyanate-utilizing alkaliphiles grown with different substrates at pH 10
| Strain(s) | Maximum cyanase activity (nmol of
NH3 mg of protein−1
min−1)a
|
||
|---|---|---|---|
| Growth without CNS− | Growth with CNS− as N source | Growth with CNS− as energy source | |
| AGCNS | 20 (acetate) | 890 (acetate) | |
| ALRh | 100 (thiosulfate) | 625 (thiosulfate) | |
| ARh 1, ARh 2, ARh 3 | 0 (thiosulfate) | 0 | 0 |
| ARh 4 | 420 (thiosulfate) | 1,890 (thiosulfate) | 35 |
a
Cyanase activity was measured in 0.1 M HEPES–NaOH–10 mM NaHCO3 (pH 8.0) with 2 mM cyanate; the incubation time was 5 to 30 min, and the protein concentration was 0.03 to 0.1 mg ml−1.