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. 2022 Jun 23;9:896416. doi: 10.3389/fvets.2022.896416

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Copyright © 2022 Shen, Wang, Huang, Lin, Zhang, Liu, Zhang and Liao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cleaved probe-based reverse transcription loop-mediated isothermal amplification (CP-RT-LAMP) assay optimization (A) Temperature: (a) 63°C, (b) 61°C, (c) 62°C, (d) 60°C, (e) 64°C, and (f) 65°C. (B) Concentration of dNTPs: (a) 2.4 mM, (b) 2 mM, (c) 1.6 mM, (d) 1.4 mM, and (e) 1.2 mM. (C) Concentration of MgSO₄: (a) 8 mM, (b) 6 mM, (c) 4 mM, and (d) 2 mM. (D) Concentration of the loop primer probe: (a) 0.08 μM, (b) 0.12 μM, (c) 0.04 μM, and (d) 0.16 μM.