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Limit of detection of cleaved probe-based reverse transcription loop-mediated isothermal amplification (CP-RT-LAMP) assay. Serially diluted standard RNA of porcine deltacoronavirus (PDCoV) was used at the following concentrations: (a) 2.5 × 106 copies/μL, (b) 2.5 × 105 copies/μL, (c) 2.5 × 104 copies/μL, (d) 2.5 × 103 copies/μL, (e) 2.5 × 102copies/μL, (f) 2.5 × 101 copies/μL, (g) 2.5 × 100 copies/μL, (h) negative control.
