Fig. 10.

Inhibition of mitochondrial function diminishes the apigenin-conferred protection from OA-induced lipid accumulation.

Huh7 cells were pretreated with OA (125 μg/mL) for 24 h, autophagy inhibitor with or without mitochondrial inhibitors (oligomycin, Oligo, 0.5 μM, or Rotenone, 0.5 μM) for 2 h, and then with or without apigenin (20 μM) for an additional 18 h. (A and C) Representative images of oil red O staining of Huh7 cells. (B and D) Representative images of the intracellular lipid accumulation and thee quantitative results of Nile red staining. Data are the mean ± SEM from five independent experiments. *P < 0.05 vs. the OA-treated group; ^#P < 0.05 vs. the OA + apigenin-treated group.