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Apigenin induces autophagy in Huh7 cells.
(A–F) Huh7 cells were pretreated with OA (125 μg/mL) for 24 h and then apigenin (20 μM) for the indicated concentrations. Western blot analysis of protein levels of Beclin1, ATG5, ATG7, LC3, SQSTM1, and α-tubulin. Data are the mean ± SEM from 5 independent experiments. *P < 0.05 vs. the 0 μM group.
