Fig. 4.

Enhancement effect of UA plus RAGE siRNA treatment on RAGE, pP65, and MDR1 protein downregulation. MIA PaCa-2 ^GEMR cells were transfected with an empty vector (named Vehicle cells) or a RAGE siRAGE (named siRAGE cells). Vehicle cells and siRAGE cells treated with or without 50 μM UA and 0.5 μM GEM for 24 h, respectively. (A) RAGE, pP65, and MDR1 protein expression was analyzed by Western blotting. The relative protein levels of (B) RAGE, (C) pP65, and (D) MDR1 are shown as the mean ± SD of three independent measurements. *p < 0.05 vs. untreated vehicle cells, ^$p < 0.05 vs. GEM-treated vehicle cells.