Fig. 5.

GSDMD inhibition prevents cell damage induced by NETs associated with SARS-CoV-2 infection. Blood isolated neutrophils (1 × 10⁶ cells) from healthy donors, pretreated, or not, with disulfiram (30 µM) were incubated, or not, with SARS-CoV-2 (n = 36). After 1 h, these neutrophils were washed twice and co-cultured with lung epithelial cells (A549, 2 × 10⁵ cells) or endothelial cells (HUVEC, 2 × 10⁵ cells) previously stained with viability dye for 24 h at 37 °C. A Representative dot plots of FACS analysis for viability dye + A549 cells. B Frequency of viability dye + A549 cells. C Representative dot plots of FACS analysis of viability dye + HUVEC. E Frequency of viability dye + HUVEC cells. Data are representative of at least two independent experiments and are shown as mean ± SEM (*or ^# p < 0.05, one-way ANOVA followed by Tukey’s test in B and D)