Fig. 3. Multiplex immunofluorescence imaging of lung tissues from naïve, WT-SARS-CoV-2, or MA-SARS-CoV-2-infected 129S1 mice immunostained for SARS-CoV-2 N protein (green), ACE2 (red), and SARS-CoV-2 Spike protein (blue).
AW: airway. The orange (left and center) and red (right) box is magnified showing N-Protein and Ace2 staining. White arrowheads indicate Ace2+/SARS-CoV-2 N+/SARS-CoV-2 S+ cells. Scale bar: 70, 30, and 10 μm as indicated in the figure panels. N = 4 mice per group and one representative image per group is shown.