Fig. 4. Multiplex immunofluorescence imaging of lung tissues from naïve, SARS-CoV-2, or MA-SARS-CoV-2-infected 129S1 mice at 3 days post-infection immunostained for Siglec-F (teal), Ly6G (magenta), Epcam (orange), SARS-CoV-2 N protein (green), CD169 (blue), Net/H3 (red), Ki-67 (white), and CD11c (yellow).
(Left to right) The red box is magnified showing SARS-CoV-2 N-Protein with CD169 + AM, Neutrophils, Ki-67+ AM, and Siglec-F + AM. White arrowheads indicate SARS-CoV-2+/Ly6G+/Net/H3+ cells, red arrowheads indicate SARS-CoV-2+/CD169+/CD11c+ cells. AW: airway. Arter: arteria. Scale bar: 50 μm and 20 μm. N = 4 mice per group and one representative image per group is shown.