Table 2. Reagents and volume.

Component	Volume
Total RNA	50 ng–5 µg
Anchored Oligo (dT)18 primer (0.5 µg/µL)	1 µL
Or Random Primer (0.1 µg/1 µL)	1 µL
2x ES Reaction Mix	10 µL
EasyScript RT/RI Enzyme Mix	1 µL
gDNA Remover	1 µL
RNase-free water	Variable
Total volume	20 µL