Table 3. Comparison of the Kinetic Parameters of SaPrp to Those of Various Cysteine Proteasesa.
| Km (μM) | kcat (s–1) | kcat/Km (M–1 s–1) | |
|---|---|---|---|
| SaPrpb | 3.4 ± 1.8 | 0.26 ± 0.05 | 74,000 ± 38,000 |
| TEV protease44 | 61.0 ± 10.0 | 0.16 ± 0.01 | 2620 ± 460 |
| papain45 | 0.62 ± 0.09 | 0.0162 ± 0.000135 | 26,000c |
| cathepsin B46 | 2000 ± 400 | 0.3 ± 0.05 | 150c |
| μ-calpain47 | 20.54 ± 2.28 | 0.015 ± 0.002 | 730 ± 46 |
Values represent means and standard deviations. Abbreviation: TEV, tobacco etch virus.
Data from this work.
Standard deviation not reported.