TABLE 2.
Distribution of tetQ and of MLS- and CTnDOT-specific genes found in community and clinical Bacteroides sp. isolates
| Isolate groupc (n) | % of isolates with resistance or hybridizing to the indicated probe
|
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Tcr | CTnDOT element probesa
|
Emr | MLS and IS probesb
|
|||||||
| tetQ | CTn ends | rteB | rteC | ermF | ermG | ermB | IS4351d | |||
| Community | ||||||||||
| VPI, pre-1970 (32) | 28 | 28 | 34 | 47 | 31 | 0 | 0 | 0 | 0 | 0 |
| VPI, 1970–1980 (33) | 42 | 42 | 48 | 48 | 42 | 0 | 0 | 0 | 0 | 0 |
| WH, 1996–1997 (86) | 80 | 83 | 81 | 83 | 76 | 29 | 15 | 0 | 2 | 5 |
| Sewage, 1997 (16) | 62 | 62 | 88 | 81 | 75 | 38 | 12 | 6 | 6 | 0 |
| Clinical | ||||||||||
| VPI, pre-1970 (23) | 22 | 22 | 30 | 43 | 26 | 9 | 9 | 0 | 0 | 0 |
| Loyola VA (65) | 83 | 83 | 85 | 83 | 80 | 31 | 17 | 8 | 2 | 3 |
| WAL Cm (20) | NDe | 60 | 60 | 90 | 60 | 5 | 5 | 0 | 0 | 0 |
| WAL Tc (14) | 100 | 100 | 86 | 93 | 79 | 64 | 50 | 14 | 14 | 0 |
| Other (10) | 80 | 80 | 90 | 90 | 80 | 70 | 60 | 10 | 0 | 20 |
Genes found on a family of Bacteroides 50- to 70-kbp CTn's called the CTnDOT family (38). The sources of the DNAs used for probes are described in Table 1.
MLS genes or erm genes found by hybridization. Active genes confer resistance to all three groups of antibiotics. Several strains hybridized to two different erm probes.
Strains are described in Materials and Methods. Cmr, all strains in this group were chloramphenicol resistant. Only two other strains, one community isolate and one clinical isolate, were found to be chloramphenicol resistant. Tcr, all the strains in this group were tetracycline resistant.
IS4351 has usually been found associated with Bacteroides regular compound transposons that also contain ermF. All of the IS4351-containing strains also hybridized to the ermF probe.
ND, not done.