TABLE 3.
Summary of the Bacteroides tetQ sequence data
| Group (no. of strains) | % Identity (no. of strains) to the following tetQ allele, source, and RFLP patterna:
|
||
|---|---|---|---|
| tetQ-1, CTnDOT, A | tetQ-2, BF1126, C or D | tet-3, BF-2, B | |
| VPI, 1960–1970s (13)b | 100 (1) | 100 (1) | 100 (3) |
| >96 (12) | >98 (6) | ||
| Woods Hole community isolates (10) | >96 (10) | 99–100 (2) | 99–100 (6) |
| Clinical isolates, 1980–presentc (10) | >96 (3) | 99–100 (2) | 100 (5) |
The accession numbers for the three Bacteroides tetQ alleles are as follows: for tetQ-1, X58717; for tetQ-2, Z21523; and for tetQ-3, Y08615. They are all >96% identical to each other, and they all were isolated from Bacteroides strains containing a CTn element. The RFLP pattern for the tetQ rteA rteB rteC region is shown in Fig. 1 and 2. The highest correlation (there were exceptions) of the tetQ allele identity with the Southern blot RFLP pattern observed with the tetQ, rteA, rteB, and rteC probes is also indicated.
Ten different species are represented by these strains.
The known CTnDOT element sequences (e.g., CTnDOT, CTnERL, CTn12256) described by Nikolich et al. (27) are not included. CTnV479 is still the only tetQ sequence with <96% identity to any of the three tetQ alleles shown.