| Chemicals, peptides, and recombinant proteins |
|
| Human Fibronectin |
Oxford Biomedical Research |
SKU:GP40 |
| 4-epi tetracycline hydrochloride |
Sigma Aldrich |
37918 |
| doxycycline |
Ding et al., 2019
|
N/A |
|
| Critical commercial assays |
|
| Lipofectamine LTX plasmid transfection reagent |
ThermoFisher |
A12621 |
| RNeasy Mini Kit |
Qiagen |
74106 |
| iScript cDNA Synthesis Kit |
Bio-Rad Laboratories |
1708891 |
| AccuPrime™ PfxSuperMix |
ThermoFisher |
12344–040 |
| iQ SYBR Green Supermix |
Bio-Rad Laboratories |
1708880 |
|
| Experimental models: Cell lines |
|
| Flp-In™ T-Rex™ HEK293 cells |
ThermoFisher |
R78007 |
| gDNA cells: FLP-in T-Tex 293 + CMV-TO- Citrine-EndoSFRS1 |
This paper |
N/A |
| cDNA cells: FLP-in T-Tex 293 + CMV-TO- Citrine-cDNA of SFRS1 |
This paper |
N/A |
| synT cells: FLP-in T-Tex 293 + CMV-TO- H2B-Ceru-3'UTR |
This paper |
N/A |
|
| Oligonucleotides |
|
| RT-PCR SRSF1 isoforms forward -ACATC GACCTCAAGAATCGCCGC |
IDT DNA |
N/A |
| RT-PCR SRSF1 isoforms reverse -ATCCA GTGAGCCCTCTCCAA |
IDT DNA |
N/A |
| RT-PCR SynT isoforms forward- CACATG AAGCAGCACGACTT |
IDT DNA |
N/A |
| RT-PCR SynT isoforms reverse - ATCCA GTGAGCCCTCTCCAA |
IDT DNA |
N/A |
|
SRSF1 isoform1 gene-specific primer-TCA TCCTCCCTATCCTATCCACA |
IDT DNA |
N/A |
| RT-qPCR SRSF1 isoform1 forward - GCA GAGGATCACCACGCTAT |
IDT DNA |
N/A |
| RT-qPCR SRSF1 isoform1 reverse - GCC AAGGTTTAAAAAGCAAAGCA |
IDT DNA |
N/A |
| RT-qPCR SynT isoform 1 forward - CGG CATGGACGAGCTGTA |
IDT DNA |
N/A |
| RT-qPCR SynT isoform 1 reverse - AGTTC ACACAAACCAGGGCA |
IDT DNA |
N/A |
|
GAPDH isoform1 gene-specific primer - AGT GATGGCAT GGACTGTGG |
IDT DNA |
N/A |
| RT-qPCR GAPDH forward - GGTGTGAACC ATGAGAAGTATGA |
IDT DNA |
N/A |
| RT-qPCR GAPDH reverse - GAGTCCTTCC ACGATACCAAAG |
IDT DNA |
N/A |
|
SDHA isoform1 gene-specific primer - CTC CAGTGCTCCTCAAAGGG |
IDT DNA |
N/A |
| RT-qPCR SDHA forward - AGAGGGAGGC ATTCTCATTAAC |
IDT DNA |
N/A |
| RT-qPCR SDHA reverse - ACCGAGACAC CACATCTCTA |
IDT DNA |
N/A |
|
| Recombinant DNA |
|
| SRSF1(gDNA): CMV-TO-Citrine-EndoSFRS1 |
This paper |
N/A |
| SRSF1(cDNA): CMV-TO-Citrine-cDNA of SFRS1 |
This paper |
N/A |
| SynT: CMV-TO-H2B-Ceru-3’UTR |
This paper |
N/A |
|
| Software and algorithms |
|
| Automated acquisition software Metamorph |
Molecular Devices |
N/A |
| Easyflow |
Y. Antebi |
https://github.com/AntebiLab/easyflow
|
| Fiji |
Schindelin et al., 2012
|
https://imagej.net/Fiji
|
| Cell tracking |
Y. Antebi |
https://github.com/AntebiLab/EasyTrack
|
| MATLAB |
MathWorks |
N/A |
| The code and database for tissue-specific analysis (related to Figures 6 and S8–S10) |
This paper |
https://doi.org/10.5281/zenodo.6522983
|
| The code for the mathematical modeling section (related to Figures 7 and S11) |
This paper |
https://doi.org/10.5281/zenodo.6525681
|
| The deep-learning image analysis code |
Darvin Yi |
https://github.com/yidarvin/FirstAid
|
|
| Other |
|
| 24-well 10 mm diameter glass No. 1.5 coverslip plates |
MatTek Corp |
P24G-1.5–10-F |
