Figure 1.

Inhibition of cell proliferation of (A) human A431 cells and (B) murine ASZ001 cells by hydroxy-derivatives of vitamin D3 in comparison with 1,25(OH)₂D3 (positive) and ethanol (negative) controls. Cells were seeded in 96-well plates and treated with the listed secosteroids for 24 h and cell proliferation assessed using the MTS assay. Data are shown as means ± SEM (n=6) with ^****P<0.0001; ^***P<0.001; ^**P<0.001; ^*P<0.05 by (A) one way ANOVA with Dunnett's multi comparison post-hoc test or (B) Student's t-test. The concentrations of the secosteroids used are shown on the x axis.