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. 2022 Jul 6;14(1):2096989. doi: 10.1080/19490976.2022.2096989

Figure 2.

Figure 2.

Depletion of gut microbe aggravates HSE. (a) Representative pictures of ceca from antibiotic-treated (ABX) and ctrl mice, with ruler for scaling. (b) Survival rates of Ctrl and HSV-1 infected normal or ABX-treated mice (log-rank test). n = 8–10 mice per group. (c) The mRNA expression levels of viral gene ICP0, UL47 and UL27 in olfactory bulb (OB), cerebral cortex (CC), and pons/medulla oblongata/cerebellum (P/M/C) derived from HSV-1 infected normal or ABX-treated mice. (d) Immunohistochemistry assay was performed to detect HSV-1 viral load in OB, CC, and P/M/C derived from HSV-1 infected normal or ABX-treated mice. Scale bar, 100 μm. (e) Normal or ABX-treated mice were infected with EGFP-HSV-1, and fluorescent images were captured by the Bruker Small Animal Optical Imaging System. Left panel: representative figures of EGFP-HSV-1 distribution in brain. Right panel: quantitative analysis of fluorescence intensity of EGFP-HSV-1. *p < .05, **p < .01 or ***p < .001 versus HSV-1 group.