Figure 3.

Lack of microbes enhances immune response in mouse ob. (a) H&E staining of OB, CC, and P/M/C tissue derived from Ctrl, ABX or ABX-treated mice infected with HSV-1. (b) total RNA of tissues derived from OB, CC, and P/M/C was extracted and the mRNA expression levels of TNFα, IL-6 and CXCL2 were analyzed by qRT-PCR, respectively. ***p < .001 versus HSV-1 group. (c-d) total RNA of OB tissue was extracted and the mRNA expression profile was detected by RNA-seq. Differentially expressed genes were analyzed by KEGG. The top signaling pathways identified as enriched in HSV-1-infected mice compared to mock-infected mice (c), or HSV-1-infected ABX-treated mice when compared to normal mice (d). (e) mRNA expression profile of representative inflammatory cytokines (at least 2-fold, p < .05) in OB from normal, ABX, HSV-1, and ABX + HSV-1 mice. Color code shows linear values.