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. 2022 Jul 4;11(1):1717–1729. doi: 10.1080/22221751.2022.2093133

Figure 1.

Figure 1.

Upregulation of EGR family genes in IBV-infected cells and chicken embryos. (a) Upregulation of EGR family genes at the mRNA level in IBV-infected H1299, Vero and DF1 cells. Cells were infected with IBV (MOI∼2), and harvested at indicated time points. The levels of virus genomic RNA (gRNA) and the mRNA levels of EGR family genes (EGR1/EGR2/EGR3/EGR4) were determined by qPCR. (b) Upregulation of EGR family genes at the mRNA level in IBV-infected chicken embryos. Ten-day-old SPF chicken embryos were inoculated with 200 µL of IBV (500 PFU). At 60 hpi, chicken embryo viscera were collected. The EGR family genes (EGR1/EGR2/EGR3/EGR4) at the mRNA level as well as IBV gRNA were determined by qPCR. Shown are the results of three repeated experiments, as indicated. (c) Upregulation of EGR1 at the protein level in IBV-infected H1299 and Vero cells. H1299 and Vero cells were infected with IBV (MOI∼2), or UV-IBV. Cell lysates were harvested at the indicated time points and subjected to Western blot analysis using indicated antibodies. Sizes of protein ladders in kDa were indicated on the left.