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. 2022 Jan 28;31(13):2155–2163. doi: 10.1093/hmg/ddac009

Figure 4.

Figure 4

Expression of recombinant L3MBTL3 full-length and short transcripts obtained from blood of a patient bearing rs7740107 TT alleles. (A) Western blot of extracts from HEK293T cells transfected with constructs carrying the full-length (p-Full-V5) and short (p-Short-V5) cDNA inserts, cloned into pcDNA™6.2/V5 directional expression plasmid (p-V5). The proteins were detected with an anti-V5 antibody. Molecular weight markers are at the left of the blot. Expected molecular weight of the full-length and short protein-V5 bands are indicated at the right side of the blot. (B) Scheme of the L3MBTL3 protein (XP_006715641.1) with the functional domains as described by Xu et al. (11): binding site for RBPJ (recombination signal binding protein for immunoglobulin kappa J region, blue); two ZF (C2C2 zinc finger, yellow); three MBT (malignant brain tumour domain, red); one SAM (sterile alpha motif, green). The expected molecular weight of the produced proteins, according with the possible starting ATG translation sites are indicated.