Table 2. Measures for validated AD miRNAs.
The table reports the quantitative and statistical measures of 15 of the 17 miRNAs examined in each group, along with their Cq trend pattern.
| MiRNA | % Detected | Median ± IQR Expression Level | Median Fold Change vs NC [95% bootstrap CI] | Cq Trend Pattern | |||||
|---|---|---|---|---|---|---|---|---|---|
| NC (n=67) | MCI (n=32) | AD (n=37) | NC | MCI | AD | MCI | AD | ||
| miR-142-3p | 98.5% | 96.9% | 97.3% | 5.16 ± 1.15 | 4.93 ± 1.48 | 4.85 ± 1.26 | 0.83 [0.47,1.44] | 0.74 [0.46,1.18] | TREND |
| miR-146b-5p | 98.5% | 93.8% | 89.2% | 4.64 ± 1.10 | 4.46 ± 1.07 | 4.24 ± 1.28 | 0.81 [0.52,1.24] | 0.67 [0.44,1.02] | TREND |
| miR-146a-5p | 95.5% | 96.9% | 86.5% | 4.14 ± 1.26 | 3.94 ± 1.22 | 3.81 ± 2.24 | 0.80 [0.48,1.32] | 0.72 [0.36,1.43] | TREND |
| miR-365a-3p | 95.5% | 84.4% | 86.5% | 4.18 ± 1.52 | 3.58 ± 2.20 | 3.35 ± 1.23 | 0.48 [0.22,1.04] | 0.43 [0.22,0.85] | TREND |
| miR-193a-5p | 91.0% | 90.6% | 81.1% | 3.82 ± 1.25 | 3.62 ± 1.65 | 3.46 ± 1.24 | 0.80 [0.48,1.33] | 0.70 [0.44,1.12] | TREND |
| miR-19b-3p | 100.0% | 100.0% | 100.0% | 6.46 ± 0.99 | 6.26 ± 1.52 | 6.25 ± 0.70 | 0.90 [0.50,1.63] | 0.81 [0.57,1.14] | AD+MCI vs. NC |
| miR-484* | 100.0% | 96.8% | 100.0% | 5.60 ± 1.34 | 5.89 ± 1.66 | 5.86 ± 0.83 | 1.33 [0.84,2.10] | 1.29 [0.84,1.97] | AD+MCI vs. NC |
| miR-140-5p | 82.1% | 75.0% | 64.9% | 4.03 ± 2.04 | 4.03 ± 2.84 | 2.87 ± 3.74 | 0.94 [0.33,2.68] | 0.31 [0.11,0.89] | AD vs. MCI+NC |
| miR-331-3p | 49.3% | 46.9% | 40.5% | 0.00 ± 3.60 | 0.00 ± 3.69 | 0.00 ± 3.21 | 1.00 [0.02,51.13] | 1.00 [0.04,22.76] | AD vs. MCI+NC** |
| miR-223-3p | 100.0% | 100.0% | 100.0% | 6.43 ± 0.88 | 6.64 ± 1.20 | 6.43 ± 0.77 | 1.24 [0.85,1.82] | 1.00 [0.75,1.34] | NO TREND |
| miR-30a-3p | 97.0% | 100.0% | 91.9% | 4.85 ± 1.19 | 4.69 ± 1.49 | 4.77 ± 1.04 | 0.80 [0.44,1.47] | 0.92 [0.58,1.47] | NO TREND |
| miR-378a-3p | 68.7% | 75.0% | 78.4% | 3.97 ± 4.58 | 4.41 ± 3.82 | 4.17 ± 1.64 | 1.53 [0.42,5.59] | 1.22 [0.44,3.43] | NO TREND |
| miR-584-5p | 100.0% | 100.0% | 97.3% | 3.43 ± 0.56 | 3.54 ± 0.79 | 3.40 ± 0.83 | 1.15 [0.88,1.50] | 0.97 [0.70,1.35] | NO TREND |
| miR-597-5p | 95.5% | 96.9% | 97.3% | 2.38 ± 1.66 | 2.01 ± 1.59 | 2.50 ± 1.27 | 0.74 [0.40,1.34] | 1.14 [0.62,2.08] | NO TREND |
| miR-15b-5p | 40.3% | 46.9% | 29.7% | 0.00 ± 3.44 | 0.00 ± 3.94 | 0.00 ± 2.89 | 1.00 [0.03,39.40] | 1.00 [0.11,8.92] | NO TREND |
*
5 of 136 samples (2 NC, 1 MCI, 2 AD) failed quality control for this miRNA.
**
The trend is visible in the normalized Cq values and in the pattern of percent detections, but not on the expression scale where censored values are set to zero.