Fig. 1. Decreased heme-binding protein 1 (Hebp1) expression under high glucose (HG) conditions. (A) Representative western blots for Hebp1 in mouse corpus cavernosum endothelial cells (MCECs) and mouse corpus cavernosum pericytes (MCPs). (B) Normalized band intensity values (n=4) were quantified by use of ImageJ software (n=4). ^***p<0.001 vs. MCEC group. The relative ratio of the MCEC group was arbitrarily set to 1. (C) Immunofluorescent staining of MCPs with antibodies against Hebp1 (green) and PDGFRβ (pericyte marker). Nuclei were labeled with the DNA dye DAPI. Scale bar indicates 100 µm. (D) The Hebp1-immunopositive area in PDGFRβ-expressing cells was quantified by use of ImageJ software (n=4). (E) Representative western blots for Hebp1 in MCPs exposed to normal glucose (NG) and HG conditions. (F) Normalized band intensity values were quantified by ImageJ software (n=4). ^***p<0.001 vs. NG group. The relative ratio of the NG group was arbitrarily set to 1. IF, immunofluorescent; WB, Western blots.