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. 2022 Jun 17;50(12):6980–6989. doi: 10.1093/nar/gkac518

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© The Author(s) 2022. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Nanopore sequencing analysis of the cDNA products generated by MRT using HOTAIR template in a time course experiment. (A) Length distribution plotting of the sequencing reads that represent the cDNA products generated at different reaction times. (B) Plotting of the average cDNA length determined by nanopore sequencing against reaction time, fit to a linear model. MRT velocity was determined as 26.6 nt/s.