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. 2022 Jun 24;50(12):6953–6967. doi: 10.1093/nar/gkac527

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© The Author(s) 2022. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — Identification of the G4 structure in the ACBP promoter. (A) Analysis of the ACBP promoter sequences. Sequences 1 kb upstream of the TSS were analysed. (B) Comparison of the PQSs of the ACBP promoters in different species and some reported G4-containing sequences (60–62) predicted by QGRS Mapper. (C–F) CD analysis of the forward ssDNA of the –257/–276 nt region of the ACBP promoter in the absence or presence of 100 mM KCl in different species. The mutated nucleotides are shown in the mutant sequence with red font. WT: wild-type strand; Mut: mutated strand; Bm: Bombyx mori; Ch: Chinese hamster; Hs: Homo sapiens; Rt: rat.