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. 2022 Jun 24;50(12):6953–6967. doi: 10.1093/nar/gkac527

Figure 3.

Figure 3.

Transcriptional regulation of BmACBP by the G4 structure. (A) Structure of the compound PDS. (B) Principle of polymerase stop assay. The G4 sequence is amplified with a complementary FAM labelled primer. The PCR product can be obtained without G4 ligand. In the presence of G4 ligand that stabilizes G4 sequences into G4 structures, therefore, the primer annealing and polymerase extension are inhibited. There are no PCR product to be obtained. (C) PCR in the PSA with different concentrations of PDS. (D) EMSA of the inhibitory effect of PDS on the binding of BmLARK to the G4 structure. (E) Immunofluorescence showing the inhibition of BmLARK binding with G4 structures by PDS in Bm12 cells. All scale bars, 1 μm. (F) The mRNA level of BmACBP in Bm12 cells after treatment with PDS. (G) Pattern of disruption of the G4 structure by ASOs. The G4 structure was disrupted by ASOs in vitro. (H) The mRNA level of BmACBP in Bm12 cells after treatment with ASOs. The data are the mean ± SEM (n = 3). *P < 0.05, n.s. (nonsignificant) = P > 0.05 (Student's t test).