FIG. 1.

Amino acid sequences of the N terminus of the 17-kDa fimbrial subunit (peptide I) and internal and C-terminal peptides II and III obtained after digestion with trypsin. The sequences of degenerate primers N7, C2, and C3, which were designed by using the codons of the corresponding underlined amino acid sequences (in italics), are shown below the peptide sequences. The amino acid sequences are numbered based on the protein sequence of X. hyacinthi shown in Fig. 5. i = inosine residues; n = A, G, C, or T; m = C or A; y = T or C; and r = G or A.