FIG. 8.
Sensitivity of the PCR assay performed with nested primers JAAN and JARA. A 5- μl portion of each serially diluted sample taken from an exponentially grown culture (approximately 3 × 108 CFU/ml) of X. hyacinthi S148 was used as a template in PCR. The detection limit was about 5 CFU/5 μl (lane 18). The arrow indicates the 229-bp amplicon from fimA. Lane M contained a 100-bp DNA ladder (Promega); the other lane designations indicate the reciprocal serial dilutions of the culture used in the 1.4% agarose gel.