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. 2001 Feb;67(2):598–607. doi: 10.1128/AEM.67.2.598-607.2001

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Copyright © 2001, American Society for Microbiology

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FIG. 9 — Specificity of the PCR with nested primers developed by using the internal sequence of fimA from X. campestris pv. vesicatoria NCPPB3240: analysis of isolates of X. vesicatoria and X. axonopodis pv. vesicatoria. Lane O, negative control; lane 1, X. vesicatoria LMG911; lane 2, X. vesicatoria LMG917; lane 3, X. axonopodis pv. vesicatoria LMG668; lane 4, X. axonopodis pv. vesicatoria LMG905; lane 5, X. vesicatoria LMG920; lane 6, X. vesicatoria LMG925; lane 7, X. vesicatoria NCCPB3240; lane 8, X. axonopodis pv. vesicatoria LMG910; lane 9, X. axonopodis pv. vesicatoria LMG913; lane 10, X. axonopodis pv. vesicatoria LMG922; lane 11, X. axonopodis pv. vesicatoria LMG929; lane 12, X. vesicatoria ATCC 35937; lane 13, X. vesicatoria ATCC 11551; lanes M, 100-bp ladder (Promega). The arrow indicates 202-bp X. vesicatoria-specific amplicons.