Figure 1.

An IAV replication defect in TRIM25 KO HEK293 cells can be rescued with RNA binding or ubiquitination deficient TRIM25 mutants. (A) TRIM25 WT, TRIM25ΔRBD or TRIM25ΔRING were re-integrated into HEK293 TRIM25 KO cells using the Flp-In recombinase system. LHS: domain architecture of wild-type TRIM25 and deletion mutants. Relative position of host RNA-binding domain (RBD) is shown in red. RHS: levels of proteins were compared to WT cells by western blot. (B) Cell lines were infected with IAV PR8 WT or PR8 NS1 R38K41A (MOI = 0.0001) and virus titers were assessed by the endpoint dilution assay. Whiskers represent the standard deviation (SD) from three biological replicates. Dpi—days post-inoculation. The asterisk (*) indicates P < 0.05 in mixed-effect model followed by Sidak's multiple comparison test.