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. 2022 Jun 23;50(12):7097–7114. doi: 10.1093/nar/gkac512

Figure 4.

Figure 4.

HeLa TRIM25 KO cells or Calu-1 TRIM25 KD, but not MEF TRIM25 KO maintain efficient phosphorylation of IRF-3 upon stimulation with 3p-hpRNA or IAV. (A) Western blot analysis of IRF-3 phosphorylation in HeLa WT and TRIM25 KO cells at 6 h post-transfection with 100 ng/ml 3p-hpRNA. (B) Western blot analysis of IRF-3 phosphorylation in HeLa WT and TRIM25 KO cells 24 hpi with PR8 R38K41A IAV (MOI = 15). (C) Western blot analysis of IRF-3 phosphorylation after 24 h in Calu-1 WT and TRIM25 KD cells transfected with 100 ng/ml 3p-hpRNA. (D) Western blot analysis of IRF-3 phosphorylation in Calu-1 WT and TRIM25 KD cells 24 hpi with PR8 R38K41A IAV (MOI = 15). (E) Western blot analysis of IRF-3 phosphorylation after 6 h in MEF WT and TRIM25 KO cells transfected with 100 ng/ml 3p-hpRNA.

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